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The process must be performed carefully because peptides can be sensitive to: Temperature changes Mechanical agitation pH levels Microbial contamination Repeated freeze-thaw cycles Equipment Needed for Safe Peptide Reconstitution Lyophilised peptide vial Sterile bacteriostatic water or sterile water for injection Alcohol swabs Sterile syringe and needle Protective gloves Sterile mixing vial (optional) Labels and marker Refrigerator or laboratory freezer for storage Step-by-Step Guide to Reconstituting Peptides 1

Some protocols add 0.6% acetic acid for better solubility