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The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm 2 nm

Clinical studies consistently report elevated circulating and central levels of pro-inflammatory cytokines, including interleukin-1 (IL-1), interleukin-6 (IL-6), and tumor necrosis factor- (TNF-), in patients with major depressive disorder, with cytokine concentrations often correlating with symptom severity, illness chronicity, and treatment resistance (30, 32)