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Types of Samples Cotinine can be measured in several biological specimens: Testing Methods Immunoassays (ELISA, rapid tests) Quick, inexpensive Can be qualitative (yes/no) or semi-quantitative Common in clinical and workplace screening Chromatography-based methods (GC-MS, LC-MS/MS) More accurate and quantitative Can distinguish nicotine from cotinine and other metabolites Used in research or forensic settings Interpretation of Results Cutoff values vary by lab and context

excerpt Milov, Sarah

This standard was chosen over the previously used tridecyl acetate standard because benzofuranone was found to be more stable over time and it does not elute in regions of the chromatogram where other analytes in the samples elute (especially A