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The researchers used Annexin-V FACS analysis and DNA laddering to demonstrate that Sag activated apoptosis in the sensitive cell lines in a dose- and time-dependent manner

Intracellularly, Cu acts as an essential cofactor that activates secretory enzymes (e.g., CP and LOX) is delivered to SOD1 via CCS to combat superoxide and stabilizes HIF-1 to promote VEGF expression

in the de-nitrosylation process two vicinal cysteines in the protein become oxidized while the nitrosothiol is reduced producing free nitroxyl (HNO) or NO, then Trx is efficiently reduced by the NADPH-dependent activity of Trx reductase enzyme