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Immunostaining of hNPCs and Imaging For immunostaining of treated hNPCs either on coverslips or in 24-well tissue culture plates, after 24 h of exposure, cells were washed three times with 1 phosphate-buffered saline (PBS) (VWR, Cat# VWRL0119-0500) and fixed with 4% paraformaldehyde (PFA) (Fisher Scientific, Cat# PI28908) and 4% sucrose (Fisher Scientific, Cat# A1558336) in 1 PBS for 20 min