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The liquid chromatography (LC) gradient program was: 98% B for 1 minute, followed by a linear decrease to 40% B within 5 minutes, then maintaining 40% B for 13 minutes, then returning to 98% B in 1 minute and finally 5 minutes 98% B for column equilibration

The blend is pre-mixed at a 5:1:1 ratio

Therapeutic strategies should integrate pharmacological interventions (e.g., anti-inflammatory and psychiatric medications), oral hygiene management (e.g., regular scaling and enhanced oral cleansing), psychological interventions, and microbiota modulation (e.g., probiotic administration) to create a comprehensive treatment model (Qian J