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After 5 min, the peptides were eluted with SCX Solvent B (25% MeCN, 5 mM KH 2 PO 4 , 500 mM KCl, pH 2.7) using a gradient from 0% to 50% B in 75 min followed by 50% to 100% B in 5 min and then maintained at 100% B for 10 min at a flow rate of 50 l/min (Supplementary Fig

BPC-157 reconstitution typically uses 12 mL of bacteriostatic water per 5 mg vial, producing a concentration of 2.55 mg/mL

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