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Blanco) were derived from E14.5 d embryos, SV40 T-antigen transformed, and grown in Dulbeccos modified Eagles medium (DMEM, Corning) containing 10% fetal bovine serum (VWR Life Sciences, Seradigm) and 100 g/mL penicillin (Sigma) at 37 C and 5% CO 2

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The transcription factor specificity protein 1 (SP1) has the ability to attach to the BACH1 gene's promoter region, thereby controlling its fundamental level of expression

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