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Description

Plates were read 5 min following drug addition using a PheraStar FSX equipped with a nanoBRET-compatible optic module (LUM 610 450) (BMG Labtech)

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Then, 95 L supernatant was transferred to a labeled 1.5-mL microcentrifuge tube, and 5 L of an internal standard of Sph/Cer was added

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