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Alizarin red staining Transfected SCAPs in Con-over group, Let-7c -over group, Con-low group and Let-7c -low group were respectively seeded into 24-well plates (Nunc, USA) at a density of 1 10 4 cells/well and cultured in routine media or mineralization-inducing media (MM) (containing -MEM, 6% fetal bovine serum, 100 U/mL penicillin, 100 g/mL streptomycin, 100 M ascorbic acid, 2 mM 2-glycerophosphate and 10 nM dexamethasone)

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Among these, serum/glucocorticoid-regulated kinase 1 (SGK1) expression patterns in circulating endothelial cells showed consistency between healthy individuals and CHD patients