Similar reductions have been seen in many wealthy countries, though in some poorer countries, smoking prevalence is not declining as consistently
Chan between 2016 and 2017 by indirect immunofluorescence at a 1:80 dilution using the NOVA Lite HEp-2 ANA slide with DAPI kit (Inova Diagnostics, San Diego, California, USA), with a highly specific fluorescein isothiocyanate-conjugated secondary antibody (goat anti-human IgG)
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The data, which comes from the 2011, 2012, and 2013 National Youth Tobacco surveys of middle and high school students, show that youth who had never smoked conventional cigarettes but who used e-cigarettes were almost twice as likely to have intentions* to smoke conventional cigarettes as those who had never used e-cigarettes
