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Cat #: BEK-2211-2P, Biosensis Pty Ltd., SA, Australia), ChemKine TM BDNF Sandwich ELISA (Cat #: CYT306) and Milliplex Map Human Pituitary Magnetic Bead Panel 2 - Endocrine Multiplex Assay, based on Luminex /xMAP technology (Cat #: HPTP2MAG-66K, both from EMD Millipore Corporation, Billerica, MA, USA), BDNF Emax Immuno-Assay System (Cat #: G7610, Promega Corporation, Madison, WI, USA), Quantikine human BDNF Immunoassay (Cat #: DBD00, R&D Systems, Inc., Minneapolis, MN, USA)

17 The formation of peroxynitrite occurs when superoxide radicals interact with nitric oxide, resulting in nitrotyrosine adducts within mitochondria

The mechanism by which melatonin inhibits GnRH secretion is not clear