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The active caspase-1 converts pro-IL-18 and pro-IL-1 into IL-18 and IL-1, which further induces an inflammatory response and triggers pyroptosis [74, 88]

After 7 days, cells were washed with PBS, fixed with 4% paraformaldehyde for 15 min, and stained with alcian blue 8GX solution for 30 min at RT to detect intracellular glycosaminoglycan (GAG)