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Whereas most of the included studies were varied in quality from low to high, most studies had small sample size and only two had large sample size, participants were predominantly male, used different comparison groups, done at different settings, most of the studies were done in the USA, and some of the studies did not report the related findings of NRT effect

In primates, exposure to 3 hours of an anesthetic regimen that produced a light surgical plane of anesthesia did not increase neuronal cell loss, however, treatment regimens of 5 hours or longer increased neuronal cell loss