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Different laboratories may employ varying preparation methods depending on research use, analytical techniques, and experimental design

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It is also generally advised against people having Wilson's disease or any other health issue affecting copper metabolism, because of the presence of copper-binding peptide in this formulation

It is used in the treatment and prevention of vitamin B12 deficiency in patients with conditions such as: Addisonian (pernicious) anemia Malabsorption Atrophic gastritis Prolonged treatment with metformin Small bowel bacterial overgrowth (SIBO) Total or partial gastrectomy Infection with the fish parasite Diphyllobothrium latum Helicobacter pylori infection Pancreatic dysfunction Pancreatic or bowel malignancy Dietary deficiency (e.g