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This bacteriostatic (growth-inhibiting) property, rather than a bactericidal (bacteria-killing) effect, ensures that any contaminating microbes do not multiply

Temperature excursions

A portion of the skin was fixed with 4 % paraformaldehyde and embedded in paraffin wax for immunohistochemical staining

Real-time quantitative polymerase chain reaction Total RNA was extracted from cells using the Trizol reagent (Takara Bio Inc, Japan) according to the manufacturers instructions