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To confirm the viability of the bacterial strains at the end of the experiment, the blood culture content was subcultured anaerobically for 48 hours on Columbia Agar medium supplemented with 5% sheep blood (BioMrieux)

These changes in the ultrastructure of rat testicular tissues indicated that the simulated high-altitude, low-pressure and hypoxic environment affects the tight junctions between rat Sertoli cells, mesenchymal cells and spermatogonia

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