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From each subject at least one thousand binucleated cells (BC) with well-preserved cytoplasm (500 per replicate culture) were analyzed and the frequency of micronucleated binucleated cells per 1000 binucleated cells (MNBC/1000 BC) was calculated and represented as the mean number of MNBC/1000 BC SD

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17 Subjects were studied by testing the acid in their esophagus two hours after the ingestion of a plain hamburger and a glass of ice water