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Inconsistent storage creates inconsistent dosing, which creates inconsistent results, which makes it nearly impossible to optimize your protocol because you cannot tell whether the problem is the dose or the storage

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To further clarify the fibrotic changes in NP cells, GFP +ve NP cells were cocultured with GFP ve L929 or 293T cells, and then the expression of GFP and FSP1 was examined under immunofluorescence