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Supernatants for transduction of H838 cells were prepared 24 hours after transfection by passing through a 0.45 m filter and supplementing with 8 g/ml polybrene (Sigma)
Much of the evidence that is still missing may arrive sooner than the traditional model would suggest, as AI accelerates large decentralized real-world trials that can collect human data on these interventions at a scale and pace conventional trials could not reach
SD was achieved with rotation (5 rev/min) from 14:00 to 10:00 + 1 day for 20 h
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