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Fluorescent products (lipid- and water-soluble) of lipid peroxidation were determined by spectrofluorimetric analysis at 360/430 (excitation/emission) nm 41

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Copper complexation is performed by mixing the purified tripeptide with copper(II) salts (chloride, acetate, or sulfate, depending on the protocol) at controlled pH, with the resulting complex purified by chromatography