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While the precise nature of the priming event at S114 remains unclear, our findings demonstrate that the casein kinase ortholog, KIN-19, is capable of phosphorylating adjacent serine residues in the hinge region of NHR-49

Niggemann JR, Tichy A, Eberspcher-Schweda MC, Eberspcher-Schweda E

2.13 Dot blotting analysis Proteins from 5 L of bile samples were transferred to PVDF membrane (Merck Millipore, Shanghai, China) using the Bio-Dot microfiltration apparatus according to the manufacturers instructions (Bio-Rad Laboratories, Inc., United States)