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Following normalization using the housekeeping gene, the 2Ct technique was used to calculate the fold changes in the expression levels, as described in 2.7 Detection of aflatoxin residues in eggs The egg was collected after the completion of the trial to detect the residual AFT using high-performance liquid chromatography (HPLC)

However, in this case, it is necessary to first focus on the typical nutritional requirements of the species

Liu H, Zhou L, Wang H, Wang X, Qu G, Cai J, et al

What are the criteria that define participating patients here, particularly those with cardiac, renal, diabetes, respiratory and/or behavioral health disorders