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N-terminal-SNAP-tagged ghrelin receptor (WT or with various mutations) and full-length ghrelin labeled with the dye A2 on an additional cysteine at the C-terminal end of the peptide (ghrelin-A2, synthesized by Vazyme, China) were used as previously described 41

Peptides elute from the column at different times based on their hydrophobicity

For growth hormone axis research that requires upstream GHRH pathway stimulation alongside metabolic compound investigation, our tesamorelin 10mg research guide and CJC-1295 Without DAC research guide cover the GHRH analogue research compounds mechanistically distinct from AOD-9604 and frequently studied in parallel in comprehensive metabolic research programmes