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In brief, HUVECs were lysed in Co-IP buffer containing 20 mM Tris-HCl (pH 7.5), 100 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 0.5 mM PMSF, and 0.5% protease inhibitor cocktail on ice for 20 min

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Test results are usually available within a few days

Conversely, although statistically non-significant ( p = 0.05), a trend towards a 10% decrease in diastolic blood pressure was reported in pregnant women with preeclampsia during a recent dose escalation study of activated broccoli extract (equivalent to 32 or 64 mg sulforaphane), regardless of sulforaphane dose (Langston-Cox et al., 2021)