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To fully confirm the mechanism behind CS-mediated increased biofilm we tested the biofilm forming capacity of three mutants known to be important in this process, in the presence and absence of 50% CS-TSB: an Agr mutant (JE2 agrB ::Tn) where the majority of cell surface proteins are repressed following activation of Agr 54,55 , a previously determined biofilm promoting surface protein, fibronectin binding protein A 56 , FnBPA (JE2 fnbA ::Tn) and sortase A (JE2 srtA ::Tn), an enzyme responsible for anchoring surface proteins to the cell wall 57