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Briefly, DE cells in the second 50-ml conical tube were resuspended in Hepatic Specification Medium, composed of Hepatocyte Differentiation Basal Medium supplemented with 100 ng/ml HGF, 1% DMSO as well as 10 M Y-27632 and seeded onto Matrigel pre-coated 12-well or 24-well plates, at a seeding density of 8x104 cells/cm2

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