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First, the solenogaster-specific primers 16Sarl_Solenos and 16SbrH_Solenos (McCutcheon et al., 2022), which yield an amplicon around 480 bp in length and were modified from primers by (Palumbi, 1996), were used with the following cycling parameters: 1 min at 95 C, (20 s at 95 C, 15 s at 60 C, 30 s at 72 C) x 35 cycles, 7 min at 72 C and finally cooling at 10 C

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