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Immunostaining was performed by seeding cells on glass coverslips, fixing with paraformaldehyde, permeabilizing with Triton X-100, and then incubating with primary anti-NF-B p65 antibody, followed by Alexa Fluor 546-conjugated secondary antibody

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Alcohol intake and risk of incident gout in men: a prospective study

Thus, careful consideration of patient groups and inflammatory status is essential when employing prolonged antifungal regimens