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The basic formula Concentration (mg/mL) = Total peptide (mg) Water volume (mL) For a 70mg vial with 3mL water: 70 3 = 23.33 mg/mL Volume to draw (mL) = Desired dose (mg) Concentration (mg/mL) For 2.33mg dose at 23.33 mg/mL: 2.33 23.33 = 0.10mL = 10 units on an insulin syringe Common vial sizes and calculations 42mg vial with 1.8mL water: Concentration: 23.33 mg/mL Standard dose (2.33mg): 10 units Conservative dose (1.165mg): 5 units Vial provides approximately 18 doses at standard protocol 70mg vial with 3mL water: Concentration: 23.33 mg/mL Standard dose (2.33mg): 10 units Conservative dose (1.165mg): 5 units Vial provides approximately 30 doses at standard protocol 70mg vial with 2mL water (higher concentration): Concentration: 35 mg/mL Standard dose (2.33mg): 6.7 units (round to 7) Conservative dose (1.165mg): 3.3 units (round to 3) Smaller volumes make precise measurement more difficult The SeekPeptides peptide calculator automates these calculations for any vial size and desired dose

Maas E, Robin DA, Austermann Hula SN, Freedman SE, Wulf G, Ballard KJ, et al

A Facile one-pot Synthesis of water-soluble CQDs for the Evaluation of Their Anti-amyloidogenic Propensity

BPC-157 vials typically contain 5mg and are reconstituted for twice-daily dosing at microgram-level amounts