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For metabolomics studies, the liver lobes were collected, weighed, snap frozen in liquid nitrogen, and then stored at 80 C

They are typically standardised to 7080% silymarin and contain 200400 mg silymarin per daily dose

Research suggests it acts through modulation of several interconnected signaling pathways simultaneously a broad mechanism of action that helps explain its observed multisystemic effects in laboratory models, and that also makes it challenging to evaluate through conventional single-target drug development frameworks

For example, it can prevent LPS from causing oxidative stress in the jejunum by activating the MAPK/Nrf2 signaling pathway