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Despite the inherent resistance of EMS tissue to ferroptosis, studies have shown that Erastin can still effectively induce ferroptosis in EMS lesions, leading to tissue atrophy and cell death

The samples were centrifuged at 1000 rpm for 3 min using a KUBOTA 3520 (KUBOTA, Tokyo, Japan), and absorbance was measured at 595 nm using a GeneQuant 1300 spectrophotometer (Biochrome, Cambridge, UK)

Loss of SIRT2, or expression of a catalytically inert H187Y mutant, increases global and site-specific lactylationmost prominently at H4K8lawhich in turn activates oncogenic transcripts such as SERPING1 and TRPV4, thereby accelerating proliferation and migration in SH-SY5Y cells