We combine orthogonal analytical approaches to confirm composition, assess purity, and provide structure-relevant evidence that supports metabolite assignment, method development, or repeat synthesis decisions
Collagen was enzymatically hydrolyzed using alcalase, neutrase, and flavorzyme, producing peptides named ACP, NCP, and FCP, respectively
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This study was supported by National Key R&D Program of China (2023YFC2509100), Science and Technology Commission of Shanghai Municipality (23Y31900400), National Natural Science Foundation of China (82370984 & 82370980), Natural Science Foundation of Shanghai (22ZR1437600), Shanghai's Top Priority Research Center (2022ZZ01017), and Dominant disease biological sample project of the Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine (YBKA202201)