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Ce-DAB2 staining and preparation of Drosophila larval CNS for EM The dissected flies central brain and VNC were rinsed (five times for 3 min each) with 0.15 M sodium cacodylate buffer pH 7.4 containing 2 mM CaCl 2 on ice and blocked with 20 mM glycine, 50 mM potassium cyanide and 50 mM aminotriazole in 0.15 M sodium cacodylate buffer pH 7.4 containing 2 mM CaCl 2 for 20 min on ice

6 Canonical oxidative stress pathways are dispensable for NE protection when ETC activity decreases

Unlike standard antioxidant tests that only assess total levels, this test evaluates both reduced glutathione (GSH) and oxidised glutathione (GSSG) to calculate your GSH:GSSG ratio, a gold-standard biomarker of oxidative stress and redox balance

doi: 10.1111/jfbc.13114