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Vet Immunol Immunopathol (2006) 113:219

Dont reuse needles between access events as needle contamination bypasses the benzyl alcohol preservative at the vial entry point

For protein precipitation and metabolite extraction, 25 l of plasma and diluted urine were added to 1.5 ml Eppendorf tubes, along with 225 l of methanol and 10 l of internal standard (IS) of both substances