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Kong et al., Clin Exp Rheumatol 16: 675-681 (1998)

Then, the samples were kept for 5 min at 80 C and left at room temperature for 2 h

Collagen supplementation supports connective tissue alongside muscle protein

So people at greatest risk for deficiency include those with low stomach acid (e.g., older adults and people on proton pump inhibitors or antacids) or low IF production (known as pernicious anemia), people on medications that interfere with B12 absorption (such as metformin), people who have had gastric surgery, those with GI conditions that impair nutrient absorption (like celiac or Crohn's disease), and people on vegan and vegetarian diets