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Separate lymphocytes using Ficoll density gradient centrifugation, stained cells with the indicated markers for flow cytometry (CytoFLEX, Beckman Coulter)

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In states of obesity and metabolic dysfunction, NNMT is often overexpressed in adipose tissue, depleting nicotinamide and reducing NAD+ availability

Administration of the Si-based agent significantly alleviated UC inflammation symptoms To examine whether inflammation of the large intestine, the primary symptom of UC, was suppressed by administration of the Si-based agent, MRI of the UC model mice was performed on the 5th day after DSS administration