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cDNA was amplified in MicroAmp Optical 96-well reaction plates with SYBR Green PCR Master Mix (Applied Biosystems) on an Applied Biosystems Prism 7000 sequence detection system

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According to the hematoxylin and eosin (HE) staining, tartrate-resistant acid phosphatase (TRAP) staining, and Safranin O/Fast green (SO/FG) staining, the ankle joint tissues in the saline group exhibited extensive inflammatory cell infiltration, synovial hyperplasia, severe bone erosion, and cartilage destruction (Fig