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doi: 10.1007/s12105-022-01425-w 3 ZlatarovADrenakovaPMihaylovSZgurovaNPetkovaLIvanovKD

A series of agitated washes were then performed including Type I water for 14 hours at 4 C, 0.02% trypsin/0.05% EDTA solution for 60 mins at 37 C, 3.0% Triton X-100 for 60 minutes at room temperature, 1 M sucrose for 15 mins at room temperature, 4.0% sodium deoxycholate for 60 mins at room temperature, and 0.1% peracetic acid/4% ethanol for 120 mins at room temperature to disinfect and depyrogenate the tissues

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Free insulin-like growth factor binding protein-3 (IGFBP-3) reduces retinal vascular permeability in association with a reduction of acid sphingomyelinase (ASMase)