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Cell Stem Cell 19 (5), 628642

Less common adverse events in this meta-analysis were weight loss, infections, nausea, dysphagia, lethargy, headache, abdominal pain, irregular menstruation, drowsiness, and kidney stones

For breeding, 2 male and 1 female adult zebrafish were randomly selected from home aquariums and placed in breeding tanks (approximately at 5 pm) with a grid specifically designed to prevent egg predation by separating the adults from the embryos

Material and methods Cell culture Human hepatocellular carcinoma (HepG2) (ATCC, HB-8065) were maintained in DMEM (4.5 g/l glucose, pyruvate) (Gibco), supplemented with 10% fetal bovine serum (PAA Laboratories) and penicillin/streptomycin (25 g/ml each, Gibco) at 37 C, 5% CO 2