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Bevacizumab was the first anti-angiogenic drug used in patients with RCC, followed by VEGFR inhibitors such as sunitinib, sorafenib, pazopanib, cabozantinib, and axitinib (101)

GSH undergoes thiol-disulfide exchange in a reaction catalyzed by thiol-transferase as follows: Since this reaction is a reversible reaction, the equilibrium is determined by the redox state of the cell, which depends on the concentrations of GSH and GSSG (proportional to the log of [GSH] 2 /[GSSG]) (Lu, 1999a)

This blocking step is followed by a reducing step that converts the GSSG to GSH for quantification in the luminescent reaction