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In this study, iPSCs have been differentiated using DMEM/F12 medium supplemented with L-glutamin, heparin, and B27 for astrocytes or vasculife basal medium supplemented with FGF, acid ascorbic, hydrocortisone, glutamine, and growth factor for brain endothelial cells

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While eCOA has been used for decades, its expansion at scale has introduced new complexity, as expectations for automation and performance have outpaced the underlying processes and scientific oversight required to support them