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This regulatory relationship is inversely validated at the molecular level: the GPX4 inhibitor RSL3 potently induces ferroptosis by binding to its active site, serving as a reliable experimental tool for studying ferroptosis in AD and directly establishing a causal link between GPX4 inhibition and AD pathology (191)

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However, while selecting a suitable protocol for encapsulation, various factors are checked, such as drug/lipid ratio, sterility, drug retention, encapsulation efficiency, and drug stability [137]