E. cloni-10G SUPREME Electrocompetent Cells (DUOs) Next-Gen Sequencing coli strain by manipulating a

coli strain by manipulating a gene that controls the copy number of vectors containing ColE1 or pMB1 origins of replication (e

The purity of the T4 DNA ligase is equal or greater than 95% as judged by SDS-polyacrylamide gel electrophoresis with Coomassie blue staining

SUPREME Cells have the highest transformation efficiency available from any supplier

is supplied with 10X DNA Polymerase Buffer B

E. cloni-10G SUPREME Electrocompetent Cells (DUOs) Next-Gen Sequencing coli strain by manipulating aTable 1. E. cloni Competent Cells for Cloning and Library Construction E. cloni Cell Lines Transformation Efficiency (cfu g pUC DNA) Cloning Methylated DNA BAC, Cosmid Cloning Blue White Screening 10G SUPREME Electrocompetent >4 1010 YES YES YES without IPTG induction 10G ELITE Electrocompetent >2 1010 YES NO YES without IPTG induction 10G CLASSIC Electrocompetent >5 109 YES NO YES without IPTG induction 10GF ELITE Electrocompetent >2 1010 YES NO YES