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Cells were visualized using the confocal microscopy (Observer Z1 Confocal Spinning Disc V.2 Zeiss) and the obtained data was analyzed using Image J Software

If you see upward trends within the first four to six weeks of oral dosing, the oral formulation may not be delivering adequate blood levels for maintenance

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At 0, 3, 7, and 10 days, the tissues were fixed, and cryosections were used for in situ apoptotic staining with terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL), according to the manufacturers instructions (Servicebio, Beijing, China)