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Further assessments included eNOS, iNOS, COX-2 mRNA levels and lipid peroxidation in infarcted hearts (known to be up-regulated in isoprenaline myocardial infarction [38,39,40])

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Recommended Timing Protocols Morning Fasted Protocol (Most Common): Timing: Upon waking, before any food or caloric beverages Fasting Window: At least 8-10 hours fasted from overnight sleep Post-Injection: Wait 30-60 minutes before eating breakfast Activity: Light fasted cardio or morning routine before first meal may enhance fat mobilization Advantages: Convenient, consistent daily routine, maximizes overnight fasted state Pre-Exercise Protocol: Timing: 30-45 minutes before fasted cardio or training session Fasting Window: At least 4-6 hours since last meal Rationale: Exercise during fasted state with elevated free fatty acids may enhance fat oxidation Considerations: Not suitable for high-intensity training

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