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Following collection, adipose tissue explants were rinsed in phosphate-buffered saline (PBS) (P4474, Sigma-Aldrich, Pozna, Poland) supplemented with 1% penicillin/streptomycin (P/S) (P4333, Sigma- Aldrich, Pozna, Poland) to prevent microbial contamination and then transferred into transport medium consisting of Dulbeccos Modified Eagles Mediumlow glucose (DMEM-LG containing 1000 mg/L of glucose) (D9443, Sigma- Aldrich, Pozna, Poland) supplemented with 1% P/S

Despite being just three amino acids long, it has attracted serious attention in inflammation research for its ability to dial down inflammatory signaling in gut, skin, and immune cell models without the broader hormonal effects that come with the full MSH molecule

Using lumbar support, taking regular breaks, and alternating between sitting and standing help reduce this pressure

Nevertheless, these LOX-metabolites were measured in vivo at a single time point which is likely at their elimination phase and thus it is considered a limitation of our study