PRP is made from platelets extracted from your blood
This work was supported by the Ministry of Education and Science of the Russian Federation within the fundamental research on the subject Development of strategies for diagnostics, therapy of genital endometriosis and tumors of the female reproductive tract (registration number: AAAA-A19-119030490009-6)
Rabbit anti-4-HNE polyclonal antibodies (ab46545, Abcam, Cambridge, UK) were used at 1:100 dilution
After removing the solvents from the polar phases using an SP Genevac EZ-2 series 4.0 centrifugal evaporator, the lyophilised extracts were resuspended in 500 L of a tissue-specific phosphate buffer containing 50 mM NaHPO, 1 mM 2,2,3,3-d-trimethylsilyl-propionic acid, sodium salt (TSP-d4), 50 L of D2O, and 2 mM sodium azide (NaN3) as an antibacterial agent