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Samples were incubated overnight at 4C, rinsed 5 in 1xPBS + 0.1% Triton X-100, then incubated overnight at 4C in secondary antibody + 10% NGS in 1xPBS + 0.1% Triton X-100

Strenuous exercise has resulted in increases in the inflammatory marker C-reactive protein (CRP) to levels comparable to those seen in cardiovascular disease states 12

OConnor C, White KL, Doncescu N et al (2015) NMR structure and dynamics of the agonist dynorphin peptide bound to the human kappa opioid receptor

The Nrf2 signaling pathway responds to oxidative stress by upregulation of antioxidants like glutathione (GSH) to compensate the stress insult and re-establish homeostasis