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Extensive investigations of G6PD from G6PD-deficient cells, mostly carried out before the sequence of G6PD was known, revealed that (1) enzyme activity, even when severely reduced (sometimes to less than 1% of normal), is never completely absent and (2) enzymic properties ( K m , K i , activity on substrate analogues, and thermostability, for example) are often different from those of the normal enzyme (i.e., G6PD deficiency is associated with qualitative abnormalities)